DIFFERENT CONCENTRATIONS OF AMMONIUM NITRATE (NH4NO3) AFFECT NITROGEN METABOLISM AND THE DEVELOPMENT OF Acmella oleracea L. (Asteraceae) GROWN IN VITRO
DOI:
https://doi.org/10.61164/remunom.v1i1.3434Keywords:
Jambu, Micropropagation, Nitrogen, BAPAbstract
Nitrogen deficiency in leafy vegetables, such as Acmella oleracea, affects growth and plant metabolism, negatively impacting productivity. This study aimed to evaluate the nitrogen metabolism and development of A. oleracea cultivated in vitro under different concentrations of ammonium nitrate (NH₄NO₃). Seeds were germinated in MS medium with three sucrose concentrations (30 g L⁻¹ - T1; 20 g L⁻¹ - T2; 10 g L⁻¹ - T3) and subjected to biometric analysis after 4 weeks. No significant differences were observed in the biometric variables for the number of leaves and root length, except for shoot length and fresh weight in the medium with 30 g L⁻¹ of sucrose. In the micropropagation stage, shoot tips were cultivated in basic MS medium (T1), MS with ½ NH₄NO₃ (T2), and MS with 2x NH₄NO₃ (T3), all supplemented with 0.4 mg L⁻¹ of BAP. After 4 weeks, biometric measurements and biochemical analyses were performed. T1 exhibited critical development, while T1 and T3 showed callus formation at the base of the explants, hindering root formation. In T3, a significant increase was observed in nitrate (26.52%), free ammonium (150.68%), and amino acid (94.12%) levels, accompanied by a 20.7% reduction in total soluble carbohydrates. The use of 0.4 mg L⁻¹ of BAP caused toxicity in A. oleracea plants. Increased nitrogen concentrations in the form of NH₄NO₃ may lead to reduced carbon metabolism. Therefore, appropriate ammonium nitrate concentrations should be adjusted to optimize the development of A. oleracea cultivated in vitro.
References
AKTAR, Asma et al. Pharmacological and phytochemical review of Acmella oleracea: a comprehensive analysis of its therapeutic potential. Discover Applied Sciences, [S. l.], v. 6, n. 412, 2024.
ALMEIDA, Susan P. et al. In vitro culture of jambu with different growth regulators. Horticultura Brasileira, [S. l.], v. 38, n. 2, p. 134-138, 2020.
CASSIM, Bruno Maia A. R. et al. Nitrogen: from discovery, plant assimilation, sustainable usage to current enhanced efficiency fertilizers technologies – A review. Revista Brasileira de Ciência do Solo, [S. l.], v. 48, e0230037, 2024.
CATALDO, D. A. et al. Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Communications in Soil Science and Plant Analysis, v.6, p.71-80, 1975.
CORPES, Rosana S.; SANTOS, Alberdan S. Influência dos reguladores de crescimento 2,4-D e BAP associados aos agentes solidificantes ágar e phytagel na indução de calos de Crinum americanum L. (Amaryllidaceae). Research, Society and Development, [S. l.], v. 10, n. 12, e299101220378, 2021.
DUBOIS, M. et al. Colorimetric method for determination of sugars and related substances. Anal. Chem., v. 28, p. 350–356, 1956.
FELKER, P. Microdetermination of nitrogen in seed protein extracts with the Salicylate-Dichloroisocyanurate color reaction. Anal. Chem., v. 49, p. 1080. 1977.
FERNANDES, Manlio S.; SOUZA, Sonia R.; SANTOS, Leandro A. Nutrição mineral de plantas. 2. ed. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2018.
FERREIRA, Silvia M. M. et al. Jambu varieties performance under shading screens. Rev. Ceres, Viçosa, v. 68, n. 5, p. 390-395, 2021.
GONÇALVES, Mayra J. et al. Rápida produção de mudas de pitaia (Hylocereus undatus, Cactaceae) por meio da técnica da micropropagação. Acta Biológica Catarinense, v. 7, n. 1, p. 75-81, 2020.
GUIMARÃES, Ana Clara B. et al. Potencial terapêutico do espilantol na Acmella oleracea (jambu): uma revisão da literatura. Revista Multidisciplinar do Nordeste Mineiro, [S. l.], v. 13, p. 1-7, 2023.
KAY, Matthew; WOBBROCK, Jacob O. Package ‘ARTool’. CRAN Repository, p. 1-13, 2016. Disponível em: <https://www.scirp.org/pdf/AM_2014110516415463.pdf> Acesso em: 28 nov., 2024.
KERBAUY, Gilberto Barbante. Fisiologia Vegetal. 3 ed. Porto Alegre: Guanabara Koogan, 2019.
KULUS, Dariusz. Application of synthetic seeds in propagation, storage, and preservation of Asteraceae plant species. In: FAISAL, M.; ALATAR, A. Synthetic Seeds. 1. ed. Springer: Cham, 2019.
LAMEIRA, Osmar A.; RODRIGUES, Simone M.; SOUZA, Isis N. G.; CAMPELO, Meiciane F.; MOREIRA, Ruanny K. V. P. P.; SILVA, Ana C. B.; SANTOS, Mila C. A.; RAMIRES, Allan C. S. Efeitos de diferentes concentrações de nitrato de amônio e nitrato de potássio na micropropagação de Physalis angulata L. Brazilian Journal of Development, Curitiba, v. 6, n. 11, p. 85090-85097, 2020.
LI, Huayi. Nutrients translocation & plant growth in tissue culture. Wageningen: Wageningen University & Research, 2020.
LIU, Xiujie; HU, Bin; CHU, Chengcai. Nitrogen assimilation in plants: current status and future prospects. Journal of Genetics and Genomics, v. 49, n. 5, p. 394-404, 2022.
MORAES, Francielle Kern et al. Morfogênese in vitro de plântulas de Cattleya intermedia Graham ex Hook (Orchidaceae) em diferentes concentrações de meio de cultura basal. Research, Society and Development, [S. l.], v. 12, n. 11, e48121143650, 2023.
MURASHIGE, T; SKOOG, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, v.15, p.473-497, 1962.
OLIVEIRA, Kívia S.; FREIRE, Fúlvio A. M.; ALOUFA, Magdi A. I. Influência de reguladores de crescimento e do tipo de explante na morfogênese in vitro de Hancornia speciosa Gomes. Revista Desafios, Palmas, v. 6, n. 4, p. 60-74, 2019.
PINHEIRO, Marcos V. M. et al. Modificações no meio de cultura, fotoperíodo e tempo de cultivo afetam o alongamento e enraizamento in vitro de bananeira cv. Pacovan. Nativa, Sinop, v. 6, n. 1, p. 27-32, 2018.
PRITI, Savant B.; MANJUSHA, Kareppa S. A systemetic and scientific review on the Acmella oleracea and its traditional medical and pharmacological uses. Asian Journal of Pharmaceutical Research, [S. l.], v. 12, n. 1, p. 71-75, 2022.
R CORE TEAM. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria, 2023. Disponível em: <https://www.Rproject.org/> Acesso em: 28 nov., 2024.
RELVAS, Rikelme M. S. et al. Aspectos agronômicos, uso e importância do jambu (Acmella oleracea L.): uma revisão. Scientific Electronic Archives, [S. l.], v.17, n.6, 2024.
SHAH, Durdana et al. Sugar regulates plant growth and development under in vitro conditions. In: KHAN, M. I. R. et al. Plant Signaling Molecules. 1. ed. Elsevier: Woodhead Publishing, p. 257-268, 2018.
SILVA, Andressa O. et al. Effect of shading and growing season on the agronomic performance of jambu. Pesq. Agropec. Trop., Goiânia, v. 53, e77257, 2023.
SONG, Jinnan; YANG, Jingli; JEONG, Byoung Ryong. Growth, quality, and nitrogen assimilation in response to high ammonium or nitrate supply in cabbage (Brassica campestris L.) and lettuce (Lactuca sativa L.). Agronomy, v. 11, n. 12, p. 2556, 2021.
STEIN, Ofer; GRANOT, David. An overview of sucrose synthases in plants. Frontiers in Plant Science, [S. l.], v. 10, n. 95, 2019.
TOLEDO, Jacks A.; BIASI, Luiz A. Multiplicação e enraizamento in vitro da amoreira preta cv. xavante. Cultura Agronômica, Ilha Solteira, v. 27, n. 3, p. 328-339, 2018.
WEATHERBURN, M. W. Phenol-hypochlorite reaction for determination of ammonia. Anal. Chem., v. 39, p. 971–974, 1967.
YEMM, E. W. et al. The determination of amino-acids with ninhydrin. Analyst, v. 80, p. 209–214, 1955.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 REMUNOM
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
