EVALUATION OF THE ANTIBACTERIAL EFFECT OF THE ESSENTIAL OIL OF Juniperus communis ASSOCIATED WITH THE ANTIMICROBIAL GENTAMICIN AGAINST STRAINS OF Klebsiella pneumoniae
DOI:
https://doi.org/10.61164/rmnm.v8i1.3832Keywords:
Anti-Bacterial Agents, Phytotherapy, Phytotherapy, Microbiology, DentistryAbstract
Patients in Intensive Care Units (ICUs) are susceptible to infections due to their compromised immune system. Oral changes are common during this period, which implies a greater risk of local, respiratory and opportunistic infections. Among the main causes of infections in hospital patients is Klebsiella pneumoniae, a gram-negative bacterium found in mucous membranes and resistant to a range of routinely used antimicrobials. From this perspective, complementary therapies have emerged as an alternative for combating multi-resistant bacteria, as is the case with essential oils from medicinal plants. The aim of this study was to evaluate the antibacterial effect of Juniperus communis essential oil associated with the antimicrobial gentamicin against strains of Klebsiella pneumoniae. The research was carried out using the solid medium diffusion technique. Filter paper disks containing the antimicrobial gentamicin were placed on Muller-Hinton Agar and inoculated with the bacterial suspension and soaked in the essential oil's MIC. For each of the strains chosen, a control study was carried out to correlate the inhibition halos formed. The study showed that the association had an indifferent and antagonistic effect on most of the strains investigated. However, effective responses were seen when the essential oil was combined with the antimicrobial against the KP 104 strain. The antimicrobial potential of the Juniperus communis species should be evaluated in new studies in order to understand this therapeutic property.
Keywords: Anti-Bacterial Agents, Phytotherapy, Phytotherapy, Microbiology, Dentistry.
References
AFONSO, L. S. R.; MILER-DA-SILVA, L. L.; GARRIDO, R. G. Therapeutic strategies for infections by Klebsiella pneumoniae carbapenem resistant: a narrative review. Res, Soc and Dev, v. 11, n. 7, e46211730296, June 2022.
AL-SNAFI, A. E. Medical importance of Juniperus communis - A review. Indo Am. J. P. Sc, v. 05, n. 03, p. 1779-1792, 2018.
BIUK, L. F. Avaliação do potencial antitumoral, antioxidante e antimicrobiano do óleo essencial de Juniperus communis L. (Zimbro). 2023. Dissertação (Mestrado em Ciências Biomédicas) - Universidade Estadual de Ponta Grossa, Ponta Grossa, 2023.
BLUM, D. F. C. et al. Influência da presença de profissionais em odontologia e protocolos para assistência à saúde bucal na equipe de enfermagem da unidade de terapia intensiva: Estudo de levantamento. Rev Bras de Ter Intensiva, v. 29, n. 3, p. 391–393, jul. 2017.
CLEELAND, R.; SQUIRES, E. Evalution of new antimicrobials in vitro and in experimental animal infections. In: Lorian, V. M. D. Antibiotics in Laboratory Medicine. New York: Willians & Wilkins, p. 739-788, 1991.
CLINICAL AND LABORATORY STANDARD INSTITUTE (CLSI) - Approved standard M2-A7: Performance standards for antimicrobial disk susceptibility tests. Seventh edition. Wayne-PA, 2003.
DA SILVA, J. M. D. et al. Utilization of phytotherapics in Dentistry: integrative review.
Res Soc Dev, v. 9, n. 8, p. e209985370, 2020.
DOS SANTOS, T. B. et al. The Insertion of Dentistry in Intensive Care Units. J of Health Sci, v. 19, n. 2, p. 83–88, 2017.
FRANCO, J. B. et al. Higiene bucal para pacientes entubados sob ventilação mecânica assistida na unidade de terapia intensiva: proposta de protocolo. Arq Med Hosp Fac Cienc Med Santa Casa São Paulo, v. 59, n. 3, p. 126-31, 2014.
GIRIJA, A. S. S.; GANESH, P. S. Functional biomes beyond the bacteriome in the oral ecosystem. Jpn. Dent. Sci. Rev, v. 58, p. 217–226, 2022.
GONÇALVES, A. C. et al. Zimbro (Juniperus communis L.) as a promising source of bioactive compounds and biomedical activities: A review on recent trends. Int J Mol Sci, v. 23, n. 6, Mar. 2022.
HADACEK, F.; GREGER, H. Testing of antifungal natural products: methodologies, comparatibility of results and assay choice. Phytochemical Analyses, v.11, p. 137-147, 2000.
KALABA, V. et al. Antibacterial activity of essential oil of Juniperus communis L. Quality of Life (Banja Luka) - APEIRON, v. 18, n. 1–2, 2020.
MARTIN, R. M.; BACHMAN, M. A. Colonization, Infection, and the Accessory Genome of Klebsiella pneumoniae. Front Cell Infect Microbiol, v. 8, Jan. 2018.
MORAIS, T. M.; SILVA, A. Fundamentos da odontologia em ambiente hospitalar/UTI. 1. ed. Rio de Janeiro: Guanabara Koogan, 2015, 30 p.
NOGUEIRA, J. W. S.; JESUS, C. A. C. Higiene bucal no paciente internado em unidade de terapia intensiva: revisão integrativa. Rev Eletr Enf, v.19, 2017.
OLIVEIRA, R. A. G. et al. Estudo da interferência de óleos essenciais sobre a atividade de alguns antibióticos usados na clínica. Rev Bras de Farmacogn, v. 16, n. 1, p. 77-82, mar. 2006.
PEPELJNJAK, S. et al. Antimicrobial activity of juniper berry essential oil (Juniperus communis L., Cupressaceae), Acta Pharm, v. 55, n. 4, p. 417-422, 2005.
PERUČ, D. et al. Combined Application of Juniperus communis Essential Oil and Amikacin, Clarithromycin and Rifampicin against Mycobacterium avium and Mycobacterium intracellulare. Processes (Basel, Switzerland), v. 12, n. 1, p. 111, 2024.
RAAL, A. et al. Chemical composition of essential oil of common juniper (Juniperus communis L.) branches from Estonia. Sci Rise Pharm Sci. v.40, n. 6, p. 66–73, 2022.
ROCHA, A. L.; FERREIRA E FERREIRA, E. Odontologia hospitalar: A atuação do cirurgião dentista em equipe multiprofissional na atenção terciária. Arq Odontol, v. 50, n. 4, p. 154-160, out./dez. 2014.
SAHARMAN, Y. R. et al. Clinical impact of endemic NDM-producing Klebsiella pneumoniae in intensive care units of the national referral hospital in Jakarta, Indonesia. Antimicrob Resist Infect Control, v. 9, n. 1, 2020.
SILVA, L. O. P.; NOGUEIRA, J. M. R. Resistência bacteriana: potencial de plantas medicinais como alternativa para antimicrobianos. Rev Bras de Anál Clín, v. 53, n. 1, p. 21-27, 2021.
TORRES, S. R. et al. Alterações orais em pacientes internados em unidades de terapia intensiva. Rev Bras Odontol, Rio de Janeiro, v. 71, n. 2, p. 156-9, jul./dez. 2014.
TULIO, K. de S. C.et al. Changes in the oral microbiota profile during ICU stay: colonization by potential respiratory pathogens. Arch Health Investig, v. 7, n. 9, 2018.
UZAIRUE, L. I. et al. Global prevalence of colistin resistance in Klebsiella pneumoniae from bloodstream infection: A systematic review and meta-analysis. Pathogens, v. 11, n. 10, p. 1092, 2022.
VANDEPITTE, J. et al. Procedimentos laboratoriais em bacteriologia clínica. OMS. São Paulo: Santos, 1994, p. 87.
VASCONCELOS, M. O. Avaliação da presença de patógenos respiratórios na microbiota oral de pacientes submetidos à ventilação mecânica em unidades de terapia intensiva. 2017. Trabalho de Conclusão de Curso (Graduação em Odontologia) - Faculdade de Odontologia de Fortaleza, Universidade Federal do Ceará, Fortaleza.
WANG, G. et al. The characteristic of virulence, biofilm and antibiotic resistance of Klebsiella pneumoniae. Int J Environ Res Public Health, v. 17, n. 17, p. 932-937, Aug. 2020.
TORRES, V. P. .; CAZANE, A. L.; DERÓBIO , R. S.; OLIVERA DA CRUZ, L. A. A PROEMINÊNCIA DAS COOPERATIVAS PARA O AVANÇO DOS OBJETIVOS DE DESENVOLVIMENTO SUSTENTÁVEL . Revista Multidisciplinar do Nordeste Mineiro, [S. l.], v. 2, n. 1, 2024. DOI: 10.61164/rmnm.v2i1.2129
WASFI, R.; ELKHATIB, W.F.; ASHOUR, H.M. Molecular typing and virulence analysis of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from Egyptian hospitals. Sci Rep, v.6, Dec. 2016.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Revista Multidisciplinar do Nordeste Mineiro
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
